If your polygraph is with the NSA or CIA, which commonly use the relevant/irrelevant technique, then no, your chances on a re-test are not necessarily significantly worse. It is not uncommon for applicants with these agencies to be subjected to as many as three polygraph sessions. (By contrast, the FBI ensures that virtually all of its applicants who are offered a re-test "fail" it.)

It seems that with the relevant/irrelevant technique, the key to passing is to make no substantive admissions and to appear cooperative and sincere. I recently had the opportunity to review an NSA polygraph report, and interestingly, it included no mention of the chart readings: it was all about admissions obtained (some of which were alleged by the examinee to be mischaracterizations).

If I were in your shoes, I would focus primarily on behavioral countermeasures (regarding which, see Chapter 4 of The Lie Behind the Lie Detector) and having pre-planned, non-derogatory explanations for any reactions your polygrapher may accuse you of showing.

In addition, to familiarize yourself with the procedure, you may wish to review the chapter on the relevant/irrelevant screening "test" in the Federal Psychophysiological Detection of Deception Examiner Handbook.

In probable-lie CQTs, the question, "Do you intend to answer truthfully on these security questions?" is commonly used as a "sacrifice relevant" question and is not scored. However, according to the Federal Psychophysiological Detection of Deception Examiner Handbook, in the relevant/irrelevant screening test it is considered an "overall truth" question and seems to be used for comparison purposes:

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