The interview is here:

https://twitter.com/TuckerCarlson/status/1699543001473900670

Larry only mentions the polygraph around from the 32:23 - 32:40 mark when he speaks about losing access to his YouTube account shortly after taking a polygraph test.  That is all. Tucker Carlson does not ask Larry anything about the polygraph test.

I guess the polygraph is still not a big political issue because it only applies to a select few people and the majority of the world still think it works based on TV and movies.